Title : Development of stably transfected monolayer overexpressing the human apical sodium-dependent bile acid transporter (hASBT).

Pub. Date : 2005 Aug

PMID : 16078136






6 Functional Relationships(s)
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1 Western blot confirmed the expression of the recombinant hASBT; functionality was characterized using taurocholic acid. Taurocholic Acid solute carrier family 10 member 2 Homo sapiens
2 RESULTS: In the selected clone, hASBT-mediated taurocholate permeability across hASBT-MDCK monolayers was almost 25-fold higher with sodium, than without sodium where hASBT is not functional. Taurocholic Acid solute carrier family 10 member 2 Homo sapiens
3 RESULTS: In the selected clone, hASBT-mediated taurocholate permeability across hASBT-MDCK monolayers was almost 25-fold higher with sodium, than without sodium where hASBT is not functional. Taurocholic Acid solute carrier family 10 member 2 Homo sapiens
4 RESULTS: In the selected clone, hASBT-mediated taurocholate permeability across hASBT-MDCK monolayers was almost 25-fold higher with sodium, than without sodium where hASBT is not functional. Taurocholic Acid solute carrier family 10 member 2 Homo sapiens
5 In the presence of sodium, taurocholate and mannitol permeabilities were 23.0x10(-6) cm/sec and 2.60x10(-6) cm/s, respectively, indicating high hASBT functionality and monolayer integrity. Taurocholic Acid solute carrier family 10 member 2 Homo sapiens
6 Taurocholate uptake and inhibition kinetic parameters from hASBT-MDCK were similar to those obtained from hASBT-COS7 model, confirming hASBT functionality in hASBT-MDCK. Taurocholic Acid solute carrier family 10 member 2 Homo sapiens