Title : Mechanism of benzylsuccinate synthase: stereochemistry of toluene addition to fumarate and maleate.

Pub. Date : 2005 Jun 22

PMID : 15954762






2 Functional Relationships(s)
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1 We demonstrate that when fumarate is the cosubstrate, deuterium is transferred from toluene to the C-3 pro-(R) position of benzylsuccinate, implying a syn addition of toluene to the double bond of fumarate. Deuterium complement C3 Homo sapiens
2 However, when maleate is the cosubstrate, the addition of toluene occurs in an anti fashion, so that deuterium transfer to the C-3 pro-(R) position of benzylsuccinate is also observed. Deuterium complement C3 Homo sapiens