Title : High-performance liquid chromatography of 125I-labeled mouse epidermal growth factor radioiodinated by six different methods.

Pub. Date : 1992 May

PMID : 1582020






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1 High-performance liquid chromatography of 125I-labeled mouse epidermal growth factor radioiodinated by six different methods. Iodine-125 epidermal growth factor Mus musculus
2 Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. Iodine-125 epidermal growth factor Mus musculus
3 Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. Iodine-125 epidermal growth factor Mus musculus
4 Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. Iodine-125 epidermal growth factor Mus musculus
5 EGF preparations that had been iodinated with Chloramine T, lodogen, or lodo-beads were found mainly to consist of oxidized 125I-labeled EGF moieties. Iodine-125 epidermal growth factor Mus musculus
6 EGF preparations that had been iodinated with Chloramine T, lodogen, or lodo-beads were found mainly to consist of oxidized 125I-labeled EGF moieties. Iodine-125 epidermal growth factor Mus musculus
7 This implies that HPLC purification of these 125I-labeled EGF preparations does not yield 125I-labeled EGF preparations with ligand equivalence. Iodine-125 epidermal growth factor Mus musculus
8 However, all but one HPLC column fraction of Enzymobeads-125I-labeled EGF showed ligand equivalence. Iodine-125 epidermal growth factor Mus musculus
9 Despite the small amount of the nonequivalent component in the Enzymobeads-labeled tracer, the nonchromatographed 125I-labeled EGF preparation showed ligand equivalence. Iodine-125 epidermal growth factor Mus musculus