Title : Reversing the silencing of reporter sodium/iodide symporter transgene for stem cell tracking.

Pub. Date : 2005 Feb

PMID : 15695791






5 Functional Relationships(s)
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1 The iodide uptake of F3-NIS III cells was initially higher by up to 12.9-fold than that of nontransfected HB1.F3 cells. Iodides solute carrier family 5 member 5 Homo sapiens
2 RESULTS: As hNIS transgene was gradually silenced in F3-NIS III cells, after the eighth passage its iodide uptake was 1.9-fold higher than that of nontransfected HB1.F3 cells. Iodides solute carrier family 5 member 5 Homo sapiens
3 RESULTS: As hNIS transgene was gradually silenced in F3-NIS III cells, after the eighth passage its iodide uptake was 1.9-fold higher than that of nontransfected HB1.F3 cells. Iodides solute carrier family 5 member 5 Homo sapiens
4 5-azacytidine treatment (up to 40 micromol/L) for 24 h in F3-NIS III cells increased iodide uptake and hNIS messenger RNA (mRNA) 1.8- and 1.9-fold versus nontreated F3-NIS cells, respectively. Iodides solute carrier family 5 member 5 Homo sapiens
5 Moreover, after TSA treatment (up to 62.5 nmol/L) for 24 h, iodide uptake and hNIS mRNA in F3-NIS III cells increased 36- and 1.9-fold versus nontreated F3-NIS III cells, respectively. Iodides solute carrier family 5 member 5 Homo sapiens