Title : Directed evolution of a glycosynthase via chemical complementation.

Pub. Date : 2004 Nov 24

PMID : 15548001






1 Functional Relationships(s)
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1 Using the yeast three-hybrid assay, the glycosynthase activity of the E197A mutant of the Cel7B from Humicola insolens was linked to transcription of a LEU2 reporter gene, making cell growth dependent on glycosynthase activity in the absence of leucine. Leucine 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C