Title : Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.

Pub. Date : 2003 Nov

PMID : 14560023






12 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism. Ceramides protein tyrosine kinase 2 beta Homo sapiens
2 Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism. Ceramides protein tyrosine kinase 2 beta Homo sapiens
3 Recent evidence indicates that ceramide may regulate these responses by inhibiting the stimulus-mediated activation of protein kinase B (PKB), a key determinant of cell fate and insulin action. Ceramides protein tyrosine kinase 2 beta Homo sapiens
4 Recent evidence indicates that ceramide may regulate these responses by inhibiting the stimulus-mediated activation of protein kinase B (PKB), a key determinant of cell fate and insulin action. Ceramides protein tyrosine kinase 2 beta Homo sapiens
5 However, dissociation of the kinase complex and the attendant hormonal activation of PKB were prevented by ceramide. Ceramides protein tyrosine kinase 2 beta Homo sapiens
6 Under these circumstances, ceramide activated PKCzeta, leading to phosphorylation of the PKB-PH domain on Thr(34). Ceramides protein tyrosine kinase 2 beta Homo sapiens
7 In contrast, a PKB-PH domain with a T34A mutation retained the ability to bind PIP(3) even in the presence of a ceramide-activated PKCzeta and, as such, expression of PKB T34A mutant in L6 cells was resistant to inhibition by ceramide treatment. Ceramides protein tyrosine kinase 2 beta Homo sapiens
8 In contrast, a PKB-PH domain with a T34A mutation retained the ability to bind PIP(3) even in the presence of a ceramide-activated PKCzeta and, as such, expression of PKB T34A mutant in L6 cells was resistant to inhibition by ceramide treatment. Ceramides protein tyrosine kinase 2 beta Homo sapiens
9 In contrast, a PKB-PH domain with a T34A mutation retained the ability to bind PIP(3) even in the presence of a ceramide-activated PKCzeta and, as such, expression of PKB T34A mutant in L6 cells was resistant to inhibition by ceramide treatment. Ceramides protein tyrosine kinase 2 beta Homo sapiens
10 In contrast, a PKB-PH domain with a T34A mutation retained the ability to bind PIP(3) even in the presence of a ceramide-activated PKCzeta and, as such, expression of PKB T34A mutant in L6 cells was resistant to inhibition by ceramide treatment. Ceramides protein tyrosine kinase 2 beta Homo sapiens
11 Inhibitors of PKCzeta and a kinase-dead PKCzeta both antagonized the inhibitory effect of ceramide on PKB. Ceramides protein tyrosine kinase 2 beta Homo sapiens
12 Since PKB confers a prosurvival signal and regulates numerous pathways in response to insulin, suppressing its activation by a PKCzeta-dependent process may be one mechanism by which ceramide promotes cell death and induces insulin resistance. Ceramides protein tyrosine kinase 2 beta Homo sapiens