Title : Enzymatic properties of polyethylene glycol-modified cholesterol esterase in organic solvents.

Pub. Date : 1992 Aug

PMID : 1418688






5 Functional Relationships(s)
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1 The solvent dependency and substrate specificity of polyethylene glycol (PEG)-modified cholesterol esterase (CEH) catalyzing cholesterol ester synthesis in organic solvents were studied. Polyethylene Glycols carboxylesterase 1 Homo sapiens
2 The solvent dependency and substrate specificity of polyethylene glycol (PEG)-modified cholesterol esterase (CEH) catalyzing cholesterol ester synthesis in organic solvents were studied. Polyethylene Glycols carboxylesterase 1 Homo sapiens
3 When cholesterol and linoleic acid were used as the substrates, PEG-modified CEH synthesized cholesterol linoleate only in water-immiscible organic solvents. Polyethylene Glycols carboxylesterase 1 Homo sapiens
4 Among some solvents capable of solubilizing all of the reaction components (PEG-modified CEH, cholesterol, and linoleic acid), chloroform was most suitable for enzymatic cholesterol linoleate synthesis, and the synthetic activity for cholesterol linoleate decreased in the order chloroform, benzene, toluene, and cyclohexane. Polyethylene Glycols carboxylesterase 1 Homo sapiens
5 PEG-modified CEH synthesized various cholesterol esters with significant substrate specificity. Polyethylene Glycols carboxylesterase 1 Homo sapiens