Title : Oltipraz inhibits 3-methylcholanthrene induction of CYP1A1 by CCAAT/enhancer-binding protein activation.

Pub. Date : 2003 Nov 7

PMID : 12941938






8 Functional Relationships(s)
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Protein Name
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1 Oltipraz inhibits 3-methylcholanthrene induction of CYP1A1 by CCAAT/enhancer-binding protein activation. Methylcholanthrene cytochrome P450 family 1 subfamily A member 1 Homo sapiens
2 3-MC induced CYP1A1 in H4IIE cells in a time- and concentration-dependent manner. Methylcholanthrene cytochrome P450 family 1 subfamily A member 1 Homo sapiens
3 Oltipraz (10 microM) significantly inhibited CYP1A1 and CYP1A1-luciferase gene induction by 3-MC with no increase in AhR DNA binding. Methylcholanthrene cytochrome P450 family 1 subfamily A member 1 Homo sapiens
4 Oltipraz (10 microM) significantly inhibited CYP1A1 and CYP1A1-luciferase gene induction by 3-MC with no increase in AhR DNA binding. Methylcholanthrene cytochrome P450 family 1 subfamily A member 1 Homo sapiens
5 Overexpression of dominant-negative mutant C/EBP significantly abolished the ability of oltipraz to suppress 3-MC-inducible CYP1A1 and the CYP1A1 reporter gene expression. Methylcholanthrene cytochrome P450 family 1 subfamily A member 1 Homo sapiens
6 Consistently, C/EBPbeta overexpression blocked CYP1A1 reporter gene induction by 3-MC. Methylcholanthrene cytochrome P450 family 1 subfamily A member 1 Homo sapiens
7 These results provide evidence that oltipraz suppresses 3-MC induction of CYP1A1 gene expression and that activation of C/EBPbeta by oltipraz contributes to suppression of 3-MC-inducible AhR-mediated CYP1A1 expression. Methylcholanthrene cytochrome P450 family 1 subfamily A member 1 Homo sapiens
8 These results provide evidence that oltipraz suppresses 3-MC induction of CYP1A1 gene expression and that activation of C/EBPbeta by oltipraz contributes to suppression of 3-MC-inducible AhR-mediated CYP1A1 expression. Methylcholanthrene cytochrome P450 family 1 subfamily A member 1 Homo sapiens