Pub. Date : 2003 Oct
PMID : 12941074
13 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Induction of cyclooxygenase-2 mRNA and protein expression in human gingival fibroblasts stimulated with nicotine. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 2 | OBJECTIVES: The aim of the present study was to investigate the effects of nicotine on the expression of cyclooxygenase-2 (COX-2) mRNA gene and protein in cultured human gingival fibroblasts (HGFs). | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 3 | OBJECTIVES: The aim of the present study was to investigate the effects of nicotine on the expression of cyclooxygenase-2 (COX-2) mRNA gene and protein in cultured human gingival fibroblasts (HGFs). | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 4 | Furthermore, to elucidate whether induction of COX-2 may be associated with nicotine- induced cytotoxicity, NS-398 (a selective COX-2 inhibitor), was added to test its protective effect. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 5 | RESULTS: The exposure of quiescent human HGFs to nicotine resulted in the induction of COX-2 mRNA expression. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 6 | The levels of the COX-2 mRNAs increased about 1.5 and 2.5 fold after exposure to 2.5 and 15 mm nicotine for 2 h (P < 0.05), respectively. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 7 | Moreover, the peak of COX-2 mRNA levels induced by nicotine was 10 mm at 2 h incubation period. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 8 | Investigations of the time dependence of COX-2 mRNA expression in nicotine-treated HGFs revealed a rapid accumulation of the transcript, a signal first detectable at 30 min and diminished to control level after 8 h. In addition, 10 mm nicotine also induced COX-2 protein expression in HGFs. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 9 | Investigations of the time dependence of COX-2 mRNA expression in nicotine-treated HGFs revealed a rapid accumulation of the transcript, a signal first detectable at 30 min and diminished to control level after 8 h. In addition, 10 mm nicotine also induced COX-2 protein expression in HGFs. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 10 | Investigations of the time dependence of COX-2 mRNA expression in nicotine-treated HGFs revealed a rapid accumulation of the transcript, a signal first detectable at 30 min and diminished to control level after 8 h. In addition, 10 mm nicotine also induced COX-2 protein expression in HGFs. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 11 | Investigations of the time dependence of COX-2 mRNA expression in nicotine-treated HGFs revealed a rapid accumulation of the transcript, a signal first detectable at 30 min and diminished to control level after 8 h. In addition, 10 mm nicotine also induced COX-2 protein expression in HGFs. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 12 | CONCLUSIONS: Taken together, the activation of COX-2 expression by nicotine suggests a potential role for nicotine in the pathogenesis of smoking-associated periodontal disease. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |
| 13 | CONCLUSIONS: Taken together, the activation of COX-2 expression by nicotine suggests a potential role for nicotine in the pathogenesis of smoking-associated periodontal disease. | Nicotine | prostaglandin-endoperoxide synthase 2 | Homo sapiens |