Pub. Date : 2003 Jun
PMID : 12866625
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The potential application of the o-phthaldialdehyde (OPA) reagent for quantification of the type and extent of the reaction(s) catalysed by transglutaminase (TGase) during incubation with sodium caseinate (NaCN) was investigated. | Sodium Cyanide | transglutaminase 1 | Homo sapiens |
| 2 | The potential application of the o-phthaldialdehyde (OPA) reagent for quantification of the type and extent of the reaction(s) catalysed by transglutaminase (TGase) during incubation with sodium caseinate (NaCN) was investigated. | Sodium Cyanide | transglutaminase 1 | Homo sapiens |
| 3 | Initial studies were performed to ensure that NH3, a by-product of TGase activity, could be determined with the OPA reagent in trichloroacetic acid (TCA) supernatants of NaCN solutions. | Sodium Cyanide | transglutaminase 1 | Homo sapiens |
| 4 | The release of NH3 and the decrease in epsilon-amino groups on incubating NaCN with TGase was subsequently quantified using the OPA reagent. | Sodium Cyanide | transglutaminase 1 | Homo sapiens |
| 5 | Incubation of NaCN (4% w/v) with TGase at 23 degrees C resulted in progressive increases and decreases, respectively, in NH3 and -amino group concentration with increased incubation time. | Sodium Cyanide | transglutaminase 1 | Homo sapiens |
| 6 | These changes were dependent on TGase:NaCN. | Sodium Cyanide | transglutaminase 1 | Homo sapiens |
| 7 | It was estimated that approximately 20% of the available Lys residues in NaCN were involved in TGase-catalysed cross-links. | Sodium Cyanide | transglutaminase 1 | Homo sapiens |