Pub. Date : 2003 May 13
PMID : 12747827
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We recently identified a TOR signaling (TOS) motif in the N terminus of S6K1 and the C terminus of 4E-BP1 and demonstrated that in S6K1, the TOS motif is necessary to facilitate mTOR signaling to phosphorylate and activate S6K1. | tos | mechanistic target of rapamycin kinase | Homo sapiens |
| 2 | We recently identified a TOR signaling (TOS) motif in the N terminus of S6K1 and the C terminus of 4E-BP1 and demonstrated that in S6K1, the TOS motif is necessary to facilitate mTOR signaling to phosphorylate and activate S6K1. | tos | mechanistic target of rapamycin kinase | Homo sapiens |
| 3 | However, it is unclear how the TOS motif in S6K1 and 4E-BP1 mediates mTOR signaling. | tos | mechanistic target of rapamycin kinase | Homo sapiens |
| 4 | RESULTS: Here, we show that a functional TOS motif is required for 4E-BP1 to bind to raptor (a recently identified mTOR-interacting protein), for 4E-BP1 to be efficiently phosphorylated in vitro by the mTOR/raptor complex, and for 4E-BP1 to be phosphorylated in vivo at all identified mTOR-regulated sites. | tos | mechanistic target of rapamycin kinase | Homo sapiens |
| 5 | RESULTS: Here, we show that a functional TOS motif is required for 4E-BP1 to bind to raptor (a recently identified mTOR-interacting protein), for 4E-BP1 to be efficiently phosphorylated in vitro by the mTOR/raptor complex, and for 4E-BP1 to be phosphorylated in vivo at all identified mTOR-regulated sites. | tos | mechanistic target of rapamycin kinase | Homo sapiens |
| 6 | RESULTS: Here, we show that a functional TOS motif is required for 4E-BP1 to bind to raptor (a recently identified mTOR-interacting protein), for 4E-BP1 to be efficiently phosphorylated in vitro by the mTOR/raptor complex, and for 4E-BP1 to be phosphorylated in vivo at all identified mTOR-regulated sites. | tos | mechanistic target of rapamycin kinase | Homo sapiens |
| 7 | Consistently, overexpression of a mutant of 4E-BP1 containing a single amino acid change in the TOS motif (F114A) reduces cell size, demonstrating that mTOR-dependent regulation of cell growth by 4E-BP1 is dependent on a functional TOS motif. | tos | mechanistic target of rapamycin kinase | Homo sapiens |
| 8 | Consistently, overexpression of a mutant of 4E-BP1 containing a single amino acid change in the TOS motif (F114A) reduces cell size, demonstrating that mTOR-dependent regulation of cell growth by 4E-BP1 is dependent on a functional TOS motif. | tos | mechanistic target of rapamycin kinase | Homo sapiens |
| 9 | CONCLUSIONS: Our data demonstrate that the TOS motif functions as a docking site for the mTOR/raptor complex, which is required for multisite phosphorylation of 4E-BP1, eIF4E release from 4E-BP1, and cell growth. | tos | mechanistic target of rapamycin kinase | Homo sapiens |