Title : Uncoupled regulation of leukotriene C4 synthase in platelets from aspirin-intolerant asthmatics and healthy volunteers after aspirin treatment.

Pub. Date : 2002 Nov

PMID : 12569976






4 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 METHODS: The effect of arachidonic acid or platelet agonists on LTC4 synthase activity was investigated in platelets obtained from healthy volunteers, aspirin-intolerant asthmatics or aspirin-tolerant asthmatics after in vivo treatment or in vitro pre-incubation with aspirin. Arachidonic Acid leukotriene C4 synthase Homo sapiens
2 RESULTS: Incubation of normal platelets with arachidonic acid or collagen provoked approximately 50% reduction of platelet LTC4 synthase activity, as determined by the conversion of LTA4 to LTC4. Arachidonic Acid leukotriene C4 synthase Homo sapiens
3 Arachidonic acid-induced inhibition of LTC4 synthase activity was totally abolished in platelets collected from peripheral blood already 30 min after aspirin ingestion but was fully restored in platelets collected 3 to 7 days after the administration of aspirin. Arachidonic Acid leukotriene C4 synthase Homo sapiens
4 Similarly, LTC4 synthase activity in platelets from AIA and aspirin-tolerant asthmatics (ATA) was reduced by approximately 50% after pre-treatment with arachidonic acid in vitro. Arachidonic Acid leukotriene C4 synthase Homo sapiens