Pub. Date : 2003 Feb
PMID : 12558936
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Induction of c-fos expression by nicotine in human periodontal ligament fibroblasts is related to cellular thiol levels. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
| 2 | To investigate the molecular toxicological implications of cigarette smoking on periodontal tissue, expression of c-fos early stress response gene was examined in human periodontal ligament fibroblasts (PDLFs) after exposure to nicotine. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
| 3 | The exposure of quiescent human PDLFs to nicotine resulted in the induction of c-fos mRNA expression. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
| 4 | The levels of the c-fos mRNAs increased about 2.5 and 4.8-fold after exposure to 2.5 mm and 10 mm nicotine for 2 h, respectively. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
| 5 | Moreover, the peak of c-fos mRNA levels induced by nicotine was 5 mm at 2-h incubation period. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
| 6 | Kinetic investigations of c-fos mRNA expression in nicotine-treated cells revealed a rapid accumulation of the transcript, a significant signal first detectable after 30 min of exposure. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
| 7 | This increase was transient and the level of c-fos mRNAs returned rapidly to that of control cells by 8 h. To determine whether thiol levels were important in induction of c-fos by nicotine, we pretreated cells with the glutathione (GSH) precursor, 2-oxothiazolidine-4-carboxylic acid (OTZ), to boost thiol levels, or buthionine sulfoximine (BSO) to deplete GSH. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
| 8 | Our results demonstrate that OTZ pretreatment decreased in c-fos mRNA level and BSO pretreatment enhanced in c-fos mRNA level after exposure to nicotine. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
| 9 | These results suggest that the nicotine-dependent stress-specific expression of the c-fos gene correlates with cellular thiol levels in human PDLFs. | Nicotine | Fos proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |