Title : ACTCAT, a novel cis-acting element for proline- and hypoosmolarity-responsive expression of the ProDH gene encoding proline dehydrogenase in Arabidopsis.

Pub. Date : 2002 Oct

PMID : 12376638






8 Functional Relationships(s)
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1 ACTCAT, a novel cis-acting element for proline- and hypoosmolarity-responsive expression of the ProDH gene encoding proline dehydrogenase in Arabidopsis. Proline Methylenetetrahydrofolate reductase family protein Arabidopsis thaliana
2 ACTCAT, a novel cis-acting element for proline- and hypoosmolarity-responsive expression of the ProDH gene encoding proline dehydrogenase in Arabidopsis. Proline Methylenetetrahydrofolate reductase family protein Arabidopsis thaliana
3 We previously isolated from Arabidopsis a gene encoding Pro dehydrogenase (ProDH), a mitochondrial enzyme involved in the first step of the conversion of Pro to glutamic acid. Proline Methylenetetrahydrofolate reductase family protein Arabidopsis thaliana
4 ProDH is also induced by L-Pro and hypoosmolarity. Proline Methylenetetrahydrofolate reductase family protein Arabidopsis thaliana
5 We analyzed a DNA region that is located 5" to the transcription start site (a promoter region) of ProDH to identify cis-acting elements involved in L-Pro-induced and hypoosmolarity-induced expression in transgenic tobacco (Nicotiana tabacum) and Arabidopsis plants. Proline Methylenetetrahydrofolate reductase family protein Arabidopsis thaliana
6 We found that a 9-bp sequence, ACTCATCCT, in the ProDH promoter is necessary for the efficient expression of ProDH in response to L-Pro and hypoosmolarity. Proline Methylenetetrahydrofolate reductase family protein Arabidopsis thaliana
7 We found that a 9-bp sequence, ACTCATCCT, in the ProDH promoter is necessary for the efficient expression of ProDH in response to L-Pro and hypoosmolarity. Proline Methylenetetrahydrofolate reductase family protein Arabidopsis thaliana
8 Moreover, ACTCAT is a core cis-acting element, which we have called Pro- or hypoosmolarity-responsive element (PRE), that is necessary for L-Pro-responsive and hypoosmolarity-responsive expression of ProDH. Proline Methylenetetrahydrofolate reductase family protein Arabidopsis thaliana