Title : Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase.

Pub. Date : 2002 Jul 30

PMID : 12149134






1 Functional Relationships(s)
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1 RESULTS: In a yeast t-degron (ubiquitin-argDHFR(ts))- phosphoglycerate mutase (GPM1) fusion strain, increasing periods of exposure to the non-permissive temperature 37 degrees C, even in the presence of cycloheximide, gave decreasing function, as assessed at 23 degrees C in vivo by glucose metabolism and confirmed by immunoblot. Cycloheximide phosphoglycerate mutase Saccharomyces cerevisiae S288C