Pub. Date : 2002 Jul
PMID : 12091475
11 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Effects of desipramine treatment on norepinephrine transporter gene expression in the cultured SK-N-BE(2)M17 cells and rat brain tissue. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 2 | The antidepressant desipramine (DMI) is a selective inhibitor of norepinephrine (NE) transport that down-regulates the norepinephrine transporter (NET) protein in a concentration- and time-dependent manner in vitro. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 3 | The antidepressant desipramine (DMI) is a selective inhibitor of norepinephrine (NE) transport that down-regulates the norepinephrine transporter (NET) protein in a concentration- and time-dependent manner in vitro. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 4 | The antidepressant desipramine (DMI) is a selective inhibitor of norepinephrine (NE) transport that down-regulates the norepinephrine transporter (NET) protein in a concentration- and time-dependent manner in vitro. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 5 | The antidepressant desipramine (DMI) is a selective inhibitor of norepinephrine (NE) transport that down-regulates the norepinephrine transporter (NET) protein in a concentration- and time-dependent manner in vitro. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 6 | In this study, possible regulatory effects of DMI on NET mRNA and protein levels were investigated with the NET-expressing SK-N-BE(2)M17 cell line and rat brain tissue. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 7 | Northern blot analysis showed that incubation of the cultured cells with DMI (5-500 nm) for 3 days reduced levels of NET mRNA in both its 5.8-kb (by up to 58%) and 3.6-kb forms (to 68%), whereas incubation for 14 days increased both levels (to 40% and 100%) in a concentration-dependent manner. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 8 | In contrast, NET protein levels decreased after 3-14 days of exposure of the cells to DMI, as determined by western blotting. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 9 | Thus, in situ hybridization demonstrated initially decreased, and later increased, NET mRNA levels in locus coeruleus (LC) tissue of rats treated with DMI; whereas NET protein levels in the LC were reduced after 14 days, but unchanged after three daily DMI treatments. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 10 | Thus, in situ hybridization demonstrated initially decreased, and later increased, NET mRNA levels in locus coeruleus (LC) tissue of rats treated with DMI; whereas NET protein levels in the LC were reduced after 14 days, but unchanged after three daily DMI treatments. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |
| 11 | Thus, DMI had similar effects on NET expression in vitro and in vivo, with opposite changes in NET mRNA and protein levels, suggesting that the regulatory mechanisms involved are complex and non-congruent. | Desipramine | solute carrier family 6 member 2 | Rattus norvegicus |