Title : Isocitrate binding at two functionally distinct sites in yeast NAD+-specific isocitrate dehydrogenase.

Pub. Date : 2002 Jun 21

PMID : 11953438






3 Functional Relationships(s)
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1 Ligand-binding analyses were conducted to examine effects of residue changes in putative catalytic and regulatory isocitrate-binding sites respectively contained in IDH2 and IDH1 subunits. isocitric acid isocitrate dehydrogenase (NAD(+)) IDH2 Saccharomyces cerevisiae S288C
2 Replacement of homologous serine residues in either subunit site, S98A in IDH2 or S92A in IDH1, was found to reduce by half the total number of holoenzyme isocitrate-binding sites, confirming a correlation between detrimental effects on isocitrate binding and respective kinetic defects in catalysis and allosteric activation by AMP. isocitric acid isocitrate dehydrogenase (NAD(+)) IDH2 Saccharomyces cerevisiae S288C
3 The putative isocitrate-binding sites of IDH1 and IDH2 contain five identical and four nonidentical residues. isocitric acid isocitrate dehydrogenase (NAD(+)) IDH2 Saccharomyces cerevisiae S288C