Title : Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH* (2,2-diphenyl-1-picrylhydrazyl) free radical assay.

Pub. Date : 2000 Nov-Dec

PMID : 11395328






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1 Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH* (2,2-diphenyl-1-picrylhydrazyl) free radical assay. Luminol mitochondrially encoded cytochrome c oxidase II Homo sapiens
2 The scavenging activities of three standard antioxidants, quercetin, ascorbic acid, and trolox, were evaluated by Co(II)/ethylenediamine-tetraacetic acid (EDTA)-induced luminol chemiluminescence and the 2,2-diphenyl-1-picrylhydrazyl (DPPH*) free radical assay. Luminol mitochondrially encoded cytochrome c oxidase II Homo sapiens
3 Therefore, the aim of this study was to characterise an enzyme-free and time-independent chemiluminescence method for the assessment of the scavenging profile of compounds in a cell-free system using the Co(II)/EDTA-luminol-peroxide system. Luminol mitochondrially encoded cytochrome c oxidase II Homo sapiens
4 These results showed that the three standards were efficient and effective in inhibiting both Co(II)/EDTA-induced luminol chemiluminescence and the free radical DPPH*. Luminol mitochondrially encoded cytochrome c oxidase II Homo sapiens
5 The present study has applied a simple and precise procedure for the study of hydroxyl radical scavenging activity by Co(II)/EDTA-induced luminol chemiluminescence, and this was assessed by DPPH* free radical scavenging. Luminol mitochondrially encoded cytochrome c oxidase II Homo sapiens