Pub. Date : 2001 Jun 29
PMID : 11313335
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Thioflavin T is a fluorescent probe of the acetylcholinesterase peripheral site that reveals conformational interactions between the peripheral and acylation sites. | thioflavin T | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 2 | Here we report that thioflavin T, a fluorophore widely used to detect amyloid structure in proteins, binds selectively to the AChE peripheral site with an equilibrium dissociation constant of 1.0 microm. | thioflavin T | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 3 | The fluorescence of the bound thioflavin T is increased more than 1000-fold over that of unbound thioflavin T, the greatest enhancement of fluorescence for the binding of a fluorophore to AChE yet observed. | thioflavin T | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 4 | The fluorescence of the bound thioflavin T is increased more than 1000-fold over that of unbound thioflavin T, the greatest enhancement of fluorescence for the binding of a fluorophore to AChE yet observed. | thioflavin T | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 5 | The observation of this partial quenching of thioflavin T fluorescence is a major advance in the study of AChE for two reasons. | thioflavin T | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 6 | Second, it indicates that ligand binding to the acylation site initiates a change in the local AChE conformation at the peripheral site that quenches the fluorescence of bound thioflavin T. | thioflavin T | acetylcholinesterase (Cartwright blood group) | Homo sapiens |