Title : Induction of AP-1 binding to intron 1 of SP-A1 and SP-A2 is implicated in the phorbol ester inhibition of human SP-A promoter activity.

Pub. Date : 2000 Jul-Aug

PMID : 10914330






4 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 The phorbol ester TPA reduced SP-A1 and SP-A2 promoter activity to approximately 35% to 45% compared to that of control cells. Tetradecanoylphorbol Acetate surfactant protein A1 Homo sapiens
2 The inhibitory effect of TPA was significantly reduced upon removal of the region +64/+394 relative to the SP-A1 transcription start site. Tetradecanoylphorbol Acetate surfactant protein A1 Homo sapiens
3 Using NCI-H441 nuclear proteins, electromobility shift assay analysis showed that the intron region +309/+329 of SP-A1 and the corresponding region of SP-A2 formed sequence-specific DNA/protein complexes that were induced by TPA exposure. Tetradecanoylphorbol Acetate surfactant protein A1 Homo sapiens
4 The region +318/+324 of SP-A1 contains sequences similar to a consensus AP-1 binding site, TGACTGA (TCACTGA for SP-A2), which when mutated (TGAGAGT) prevented the formation of the TPA-induced DNA/protein complex. Tetradecanoylphorbol Acetate surfactant protein A1 Homo sapiens