Title : Novel application of S-nitrosoglutathione-Sepharose to identify proteins that are potential targets for S-nitrosoglutathione-induced mixed-disulphide formation.

Pub. Date : 2000 Jul 15

PMID : 10880356






2 Functional Relationships(s)
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1 Here, we show that GSNO-Sepharose mimicks site-specific S-glutathionylation of the transcription factors c-Jun and p50 by free GSNO in vitro. Sepharose Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens
2 Furthermore, we show that c-Jun, p50, glycogen phosphorylase b, glyceraldehyde-3-phosphate dehydrogenase, creatine kinase, glutaredoxin and caspase-3 can be precipitated from a mixture of purified thiol-containing proteins by the formation of a mixed-disulphide bond with GSNO-Sepharose. Sepharose Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens