Pub. Date : 2000 Apr 28
PMID : 10785513
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In the present study we demonstrate that cAMP-stimulating agents enhance the activity of the large-conductance, calcium-activated potassium (BK(Ca)) channel in single myocytes from coronary arteries by "cross-activation" of the cGMP-dependent protein kinase (protein kinase G, PKG). | Cyclic AMP | protein kinase cGMP-dependent 1 | Homo sapiens |
| 2 | Single-channel patch-clamp data revealed that 10 micromol/L isoproterenol, forskolin, or dopamine opens BK(Ca) channels in coronary myocytes and that this effect is attenuated by inhibitors of PKG (KT5823; Rp-8-pCPT-cGMPS), but not by inhibiting the cAMP-dependent protein kinase (protein kinase A, PKA). | Cyclic AMP | protein kinase cGMP-dependent 1 | Homo sapiens |
| 3 | Finally, the stimulatory effect of cAMP on BK(Ca) channels was reconstituted in a cell-free, inside-out patch by addition of purified PKG activated by either cGMP or cAMP. | Cyclic AMP | protein kinase cGMP-dependent 1 | Homo sapiens |
| 4 | Finally, the stimulatory effect of cAMP on BK(Ca) channels was reconstituted in a cell-free, inside-out patch by addition of purified PKG activated by either cGMP or cAMP. | Cyclic AMP | protein kinase cGMP-dependent 1 | Homo sapiens |
| 5 | In summary, findings from on-cell and cell-free patch-clamp experiments provide direct evidence that cAMP-dependent vasodilators open BK(Ca) channels in coronary myocytes by cross-activation of PKG (but not via PKA). | Cyclic AMP | protein kinase cGMP-dependent 1 | Homo sapiens |