Title : Mechanism of reaction of myeloperoxidase with nitrite.

Pub. Date : 2000 Jul 7

PMID : 10777476






5 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Mechanism of reaction of myeloperoxidase with nitrite. Nitrites myeloperoxidase Homo sapiens
2 In order to clarify if nitrite could be a physiological substrate of myeloperoxidase, we investigated the reactions of the ferric enzyme and its redox intermediates, compound I and compound II, with nitrite under pre-steady state conditions by using sequential mixing stopped-flow analysis in the pH range 4-8. Nitrites myeloperoxidase Homo sapiens
3 In order to clarify if nitrite could be a physiological substrate of myeloperoxidase, we investigated the reactions of the ferric enzyme and its redox intermediates, compound I and compound II, with nitrite under pre-steady state conditions by using sequential mixing stopped-flow analysis in the pH range 4-8. Nitrites myeloperoxidase Homo sapiens
4 At 15 degrees C the rate of formation of the low spin MPO-nitrite complex is (2.5 +/- 0.2) x 10(4) m(-1) s(-1) at pH 7 and (2.2 +/- 0.7) x 10(6) m(-1) s(-1) at pH 5. Nitrites myeloperoxidase Homo sapiens
5 Nitrite is oxidized by two one-electron steps in the MPO peroxidase cycle. Nitrites myeloperoxidase Homo sapiens