Title : Mechanisms underlying the steroidogenic synergy of insulin and luteinizing hormone in porcine granulosa cells: joint amplification of pivotal sterol-regulatory genes encoding the low-density lipoprotein (LDL) receptor, steroidogenic acute regulatory (stAR) protein and cytochrome P450 side-chain cleavage (P450scc) enzyme.

Pub. Date : 2000 Jan 25

PMID : 10687849






4 Functional Relationships(s)
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1 A maximally effective concentration of insulin (1 microg/ml) synergistically augmented LH"s dose-dependent ampilification of progesterone and cAMP accumulation; viz. Cyclic AMP insulin Homo sapiens
2 Mechanistically, insulin significantly enhanced the sensitivity of granulosa cells to LH"s drive of cAMP accumulation [ED50 for LH 61 +/- 14 ng/ml (control) vs. 10 +/- 1.0 ng/ml (insulin) (P < 0.01)]. Cyclic AMP insulin Homo sapiens
3 Insulin also augmented the maximal stimulatory effect of LH; i.e. LH efficacy rose from 6.5 +/- 0.4 to 17 +/- 1.4 (pmole cAMP/microg DNA/48 h; P < 0.001). Cyclic AMP insulin Homo sapiens
4 Insulin dose-response analysis showed that insulin alone minimally elevated basal, but significantly heightened LH"s stimulation of progesterone and cAMP accumulation at (insulin) concentrations as low as 3-10 ng/ml. Cyclic AMP insulin Homo sapiens