Title : Regulation of phenobarbital induction of the cytochrome P450 2b9/10 genes in primary mouse hepatocyte culture. Involvement of calcium- and cAMP-dependent pathways.

Pub. Date : 2000 Feb

PMID : 10672003






5 Functional Relationships(s)
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1 Regulation of phenobarbital induction of the cytochrome P450 2b9/10 genes in primary mouse hepatocyte culture. Phenobarbital cytochrome P450, family 2, subfamily b, polypeptide 9 Mus musculus
2 The cGMP-dependent protein kinase (PKG) seems to play a positive role as PKG inhibitor reduced the PB-induced level of Cyp2b9/10 mRNA. Phenobarbital cytochrome P450, family 2, subfamily b, polypeptide 9 Mus musculus
3 Firstly, measurements of intracellular Fura-2 fluorescence ratio in murine hepatocytes showed that long-term PB incubation (24 and 48 h) led to a significant increase of [Ca2+]i. Secondly, treatment with an intracellular Ca2+ chelator (BAPTA-AM) nearly completely abolished PB-induced Cyp2b9/10 expression. Phenobarbital cytochrome P450, family 2, subfamily b, polypeptide 9 Mus musculus
4 Ca2+ thus appeared to mediate PB action likely via Ca2+/calmodulin-dependent protein kinase II, as KN62, a specific inhibitor of this enzyme, also dramatically inhibited PB induction of the Cyp2b9/10 genes. Phenobarbital cytochrome P450, family 2, subfamily b, polypeptide 9 Mus musculus
5 Ca2+ thus appeared to mediate PB action likely via Ca2+/calmodulin-dependent protein kinase II, as KN62, a specific inhibitor of this enzyme, also dramatically inhibited PB induction of the Cyp2b9/10 genes. Phenobarbital cytochrome P450, family 2, subfamily b, polypeptide 9 Mus musculus