Pub. Date : 1999 Dec
PMID : 10600931
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Prostaglandins buffer ANG II-mediated increases in cytosolic calcium in preglomerular VSMC. | Prostaglandins | angiotensinogen | Rattus norvegicus |
| 2 | In order to exert an appropriate biological effect, the action of the vasoconstrictive hormone angiotensin II (ANG II) is modulated by vasoactive factors such as prostaglandins PGE2 and PGI2. | Prostaglandins | angiotensinogen | Rattus norvegicus |
| 3 | In order to exert an appropriate biological effect, the action of the vasoconstrictive hormone angiotensin II (ANG II) is modulated by vasoactive factors such as prostaglandins PGE2 and PGI2. | Prostaglandins | angiotensinogen | Rattus norvegicus |
| 4 | The present study investigates whether prostaglandins alter ANG II-mediated increases in cytosolic calcium concentration ([Ca2+]i) in vascular smooth muscle cells (VSMC) isolated from rat renal preglomerular arterioles. | Prostaglandins | angiotensinogen | Rattus norvegicus |
| 5 | Increasing cAMP levels in cultured VSMC, by using either a cell-permeable analog or inhibiting phosphodiesterase activity, mirrored the antagonistic effects of prostaglandins on ANG II-stimulated increases in [Ca2+]i. Radioimmunoassays demonstrate that ANG II (10(-7) M) stimulates production of PGI2 and PGE2; the stable prostacyclin metabolite 6-keto-PGF(1alpha) was released in 10-fold greater concentrations than PGE(2.) | Prostaglandins | angiotensinogen | Rattus norvegicus |
| 6 | Increasing cAMP levels in cultured VSMC, by using either a cell-permeable analog or inhibiting phosphodiesterase activity, mirrored the antagonistic effects of prostaglandins on ANG II-stimulated increases in [Ca2+]i. Radioimmunoassays demonstrate that ANG II (10(-7) M) stimulates production of PGI2 and PGE2; the stable prostacyclin metabolite 6-keto-PGF(1alpha) was released in 10-fold greater concentrations than PGE(2.) | Prostaglandins | angiotensinogen | Rattus norvegicus |
| 7 | Indomethacin blockade of prostaglandin production potentiated both the peak (264 to 337 +/- 26 nM) and sustained [Ca2+]i responses (95 to 181 +/- 22 nM) to ANG II. | Prostaglandins | angiotensinogen | Rattus norvegicus |
| 8 | When prostaglandin analogs were added during indomethacin treatment, the ANG II response was restored to the typical pattern. | Prostaglandins | angiotensinogen | Rattus norvegicus |
| 9 | In conclusion, we demonstrate that modulation of intracellular calcium levels is one mechanism by which prostaglandins can buffer ANG II-mediated constriction in renal preglomerular VSMC. | Prostaglandins | angiotensinogen | Rattus norvegicus |