Pub. Date : 1999 Sep 10
PMID : 10580842
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Steroid-induced conformational changes of rat glucocorticoid receptor cause altered trypsin cleavage of the putative helix 6 in the ligand binding domain. | Steroids | nuclear receptor subfamily 3, group C, member 1 | Rattus norvegicus |
| 2 | This was supported by the observation that trypsin digestion of the steroid-free R651A mutant GR gave rise to the 30-kDa meroreceptor (amino acids 518-795), which displayed wild type affinity. | Steroids | nuclear receptor subfamily 3, group C, member 1 | Rattus norvegicus |
| 3 | This 30-kDa species is thus the smallest non-associated fragment of GR possessing wild type steroid binding affinity. | Steroids | nuclear receptor subfamily 3, group C, member 1 | Rattus norvegicus |
| 4 | However, unlike the estrogen receptor or the more closely related progesterone receptor, the precise proteolytic cleavage points of both the steroid-free and -bound GR fall within regions that are predicted, on the basis of X-ray crystal structures of related receptors, to be alpha-helical and resistant to proteolysis. | Steroids | nuclear receptor subfamily 3, group C, member 1 | Rattus norvegicus |