Title : Influence of cytoplasmic pH on the aggregation and Ca2+ mobilization in rabbit platelets.

Pub. Date : 1999 Jul

PMID : 10443458






4 Functional Relationships(s)
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1 Nigericin, a K+/H+ ionophore, which can induce a decrease in pHi, at 2-10 microM in 2 min incubation reduced both platelet aggregation and an increase in cytoplasmic free Ca2+ concentration ([Ca2+]i) stimulated with thrombin or U46619. Nigericin glucose-6-phosphate isomerase Oryctolagus cuniculus
2 The reduced aggregability recovered 10 min after incubation with nigericin in parallel with an increase in pHi. Nigericin glucose-6-phosphate isomerase Oryctolagus cuniculus
3 When pHi was artificially altered during the ranges of 6.6-7.4 by treatment with nigericin in K+-rich medium, aggregation by low concentrations of thrombin was dependent on the pHi. Nigericin glucose-6-phosphate isomerase Oryctolagus cuniculus
4 When pHi was artificially altered during the ranges of 6.6-7.4 by treatment with nigericin in K+-rich medium, aggregation by low concentrations of thrombin was dependent on the pHi. Nigericin glucose-6-phosphate isomerase Oryctolagus cuniculus