Title : Human vascular smooth muscle cells express a constitutive nitric oxide synthase that insulin rapidly activates, thus increasing guanosine 3':5'-cyclic monophosphate and adenosine 3':5'-cyclic monophosphate concentrations.

Pub. Date : 1999 Jul

PMID : 10440125






6 Functional Relationships(s)
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1 Human vascular smooth muscle cells express a constitutive nitric oxide synthase that insulin rapidly activates, thus increasing guanosine 3":5"-cyclic monophosphate and adenosine 3":5"-cyclic monophosphate concentrations. Cyclic AMP insulin Homo sapiens
2 AIMS/HYPOTHESIS: Insulin incubation of human vascular smooth muscle cells (hVSMC) for 120 min increases both guanosine 3":5"-cyclic monophosphate (cGMP) and adenosine 3":5"-cyclic monophosphate (cAMP) and these effects are blocked by inhibiting nitric oxide synthase (NOS). Cyclic AMP insulin Homo sapiens
3 AIMS/HYPOTHESIS: Insulin incubation of human vascular smooth muscle cells (hVSMC) for 120 min increases both guanosine 3":5"-cyclic monophosphate (cGMP) and adenosine 3":5"-cyclic monophosphate (cAMP) and these effects are blocked by inhibiting nitric oxide synthase (NOS). Cyclic AMP insulin Homo sapiens
4 RESULTS: i) By incubating hVSMC with 2 nmol/l insulin for 0-240 min, we observed an increase of both cGMP and cAMP (ANOVA: p = 0.0001). Cyclic AMP insulin Homo sapiens
5 CONCLUSION/INTERPRETATION: Human VSMC express cNOS, which is rapidly activated by insulin with a consequent increase of both cGMP and cAMP, suggesting that insulin-induced vasodilation in vivo is not entirely endothelium-mediated. Cyclic AMP insulin Homo sapiens
6 CONCLUSION/INTERPRETATION: Human VSMC express cNOS, which is rapidly activated by insulin with a consequent increase of both cGMP and cAMP, suggesting that insulin-induced vasodilation in vivo is not entirely endothelium-mediated. Cyclic AMP insulin Homo sapiens