Title : Determination of different amino sugar 2'-epimerase activities by coupling to N-acetylneuraminate synthesis.

Pub. Date : 1999 Aug 5

PMID : 10434049






1 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 The ManNAc generated by the action of N-acetyl-D-glucosamine (GlcNAc) and UDP-GlcNAc 2"-epimerases is condensed with pyruvate through the action of N-acetylneuraminate lyase and the sialic acid released is measured by the thiobarbituric acid assay. Pyruvic Acid N-acetylneuraminate pyruvate lyase Homo sapiens