Title : Protein synthesis blockade differentially affects the degradation of constitutive and nicotinic receptor-induced tyrosine hydroxylase protein level in isolated bovine chromaffin cells.

Pub. Date : 1999 Jul

PMID : 10386968






1 Functional Relationships(s)
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1 Also absent was an effect by DMPP on the rate of degradation of TH protein because pulse-chase analysis estimated a half-life for TH of 26 +/- 5 h in DMPP-treated cells, a value that was (a) essentially the same as that estimated in control cells (29 +/- 3 h), (b) within the same range as that estimated by approach to steady state (t(1/2) = 19 +/- 4 h), which measured the decline of TH protein content from the DMPP-induced steady-state level back to the basal value during deinduction with the nicotinic antagonist hexamethonium, and (c) consistent with the time course of accumulation of TH protein to a new steady-state level in response to DMPP. Hexamethonium tyrosine hydroxylase Bos taurus