Title : Ras-specific exchange factor GRF: oligomerization through its Dbl homology domain and calcium-dependent activation of Raf.

Pub. Date : 1999 Jul

PMID : 10373510






3 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 The full-length versions of the Ras-specific exchange factors Ras-GRF1 (GRF1) and Ras-GRF2 (GRF2), which are expressed in brain and a restricted number of other organs, possess an ionomycin-dependent activation of Erk mitogen-activated protein kinase activity in 293T cells (C. L. Farnsworth et al., Nature 376:524-527, 1995; N. P. Fam et al., Mol. Ionomycin mitogen-activated protein kinase 1 Homo sapiens
2 Both mutants were impaired in their ability to mediate ionomycin-dependent Erk activity in 293T cells. Ionomycin mitogen-activated protein kinase 1 Homo sapiens
3 In the absence of ionomycin, 293T cells expressing wild-type GRF1 contained much higher levels of Ras-GTP than control cells; the increase in Erk activity induced by ionomycin in the GRF1-expressing cells also induced a concomitant increase in Raf kinase activity, but without a further increase in the level Ras-GTP. Ionomycin mitogen-activated protein kinase 1 Homo sapiens