Pub. Date : 1999 Mar 16
PMID : 10077589
10 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Estradiol induces the calcium-dependent translocation of endothelial nitric oxide synthase. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 2 | Using quantitative immunofluorescence imaging approaches, we have investigated the effect of estradiol on the subcellular targeting of endothelial nitric oxide synthase (eNOS) in bovine aortic endothelial cells. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 3 | Using quantitative immunofluorescence imaging approaches, we have investigated the effect of estradiol on the subcellular targeting of endothelial nitric oxide synthase (eNOS) in bovine aortic endothelial cells. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 4 | Within 5 min after the addition of estradiol, most of the eNOS translocates from the membrane to intracellular sites close to the nucleus. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 5 | After estradiol addition, a transient rise in intracellular Ca2+ concentration precedes eNOS translocation. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 6 | Both the Ca2+-mobilizing and eNOS-translocating effects of estradiol are completely blocked by the estrogen receptor antagonist ICI 182,780, and the intracellular Ca2+ chelator 1,2-bis-(o-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA) prevents estradiol-induced eNOS translocation. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 7 | Both the Ca2+-mobilizing and eNOS-translocating effects of estradiol are completely blocked by the estrogen receptor antagonist ICI 182,780, and the intracellular Ca2+ chelator 1,2-bis-(o-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA) prevents estradiol-induced eNOS translocation. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 8 | Both the Ca2+-mobilizing and eNOS-translocating effects of estradiol are completely blocked by the estrogen receptor antagonist ICI 182,780, and the intracellular Ca2+ chelator 1,2-bis-(o-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA) prevents estradiol-induced eNOS translocation. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 9 | Use of the nitric oxide-specific dye diaminofluorescein shows that estradiol treatment increases nitric oxide generation by endothelial cells; this response is blocked by ICI 182,780 and by the eNOS inhibitor Nomega-nitro-L-arginine. | Estradiol | nitric oxide synthase 3 | Bos taurus |
| 10 | These results show that estradiol induces subcellular translocation of eNOS by a rapid, Ca2+-dependent, receptor-mediated mechanism, and they suggest a nongenomic role for estrogen in the modulation of NO-dependent vascular tone. | Estradiol | nitric oxide synthase 3 | Bos taurus |